What is the concentration of EtBr in agarose gel?
Ethidium Bromide (EtBr), a dark red, crystalline, non-volatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. The powder is used to make a stock solution of 10 mg/mL, and the working concentration for gel-staining is 1 µg/mL.
What does a 1% agarose gel mean?
A 1% gel is 1% weight/volume (w/v). [ for example, for the larger gel, make use 0.5 g. agarose in 50 ml 1X TAE; for a 1.2% gel, add 0.36 g agarose to 30 ml final volume] 3) Heat the solution to boiling in the microwave to dissolve the agarose. Note: You should not see any beads in the solution.
What is the purpose of 1% agarose gel electrophoresis?
Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight.
How do you dilute ethidium bromide in gel electrophoresis?
Ethidium bromide is commonly used nucleic acid stain for gel electrophoresis….Ethidium Bromide Solution Preparation and Recipe
- Prepare 800 mL of distilled water in a suitable container.
- Add 10 g of Ethidium bromide to the solution.
- Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
What are the dangers of ethidium bromide?
Purpose and Applicability. Ethidium bromide is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis.
Why is ethidium bromide carcinogenic?
Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption.
What does ethidium bromide mean?
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag ( nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr, which is also an abbreviation for bromoethane.
What is the formula of ethidium bromide?
The formula of the chemical compound Ethidium bromide is C21H20BrN3