What is LysoTracker Red?
LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles. • Effective labeling of live cells at nanomolar concentrations.
Is LysoTracker deep red fixable?
LysoTracker Red can stain live cells as they can be taken up by diffusion.
What does MitoTracker Red stain?
Description. MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
What does LysoTracker stain?
LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect autophagy-associated lysosomal activity in Drosophila tissues including the fat body, midgut, salivary gland and ovary, as well as in Drosophila cell culture.
How does LysoTracker red work?
Due to its hydrophobic nature (Log P = 2.1 5), LysoTracker Red enters cells via simple diffusion. Remarkably, owing to the acidic pH of the lysosome, upon entry into the lysosome, LysoTracker Red becomes protonated and hence is markedly sequestrated in lysosomes.
Can LysoTracker be fixed?
LysoTracker-stained cells can also be fixed in paraformaldehyde followed by mounting in Vectashield mounting medium without significant loss of fluorescent signal (Cao et al., 2006).
What is MitoTimer?
MitoTimer is a novel fluorescent reporter protein that can reveal new insights into mitochondrial dynamics within cells. Coupled with organelle flow cytometry, it offers new opportunities to investigate mitochondrial subpopulations by biochemical or proteomic methods.
What is the pH of a lysosome?
With a pH ranging from ~4.5–5.0, the interior of the lysosomes is acidic compared to the slightly basic cytosol (pH 7.2). The lysosomal membrane protects the cytosol, and therefore the rest of the cell, from the degradative enzymes within the lysosome.
What is the half life of mitochondria?
Studies of cardiac mitochondria revealed an average half-life of 17 days under steady state conditions [115].
How do you measure Mitophagy?
Mitophagy can be quantified by fluorescence imaging using a mitochondria-matrix targeted Keima (mt-Keima) fluorescent protein in cells and in vivo. Similar to MitoTimer, Keima is targeted to the mitochondrial matrix via fusion with COX8 to make mt-Keima.
What are the features of the lysotracker deep red dye?
LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles. • Effective labeling of live cells at nanomolar concentrations. • Live-cell imaging.
Is the base of lysotracker red partially protonated?
A molecule of LysoTracker Red consists of a fluorophore linked to a weak base that is only partially protonated at neutral pH. Similarly to AO, it is likely that the molecule is protonated on entry into an acidic organelle and the protonated form is far less likely to cross the membrane again.
How is lysotracker used to label live cells?
Learn more LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells.
What are the features of a lysotracker probe?
Features of all LysoTracker® probes include: The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells.
