What does FSC mean in flow cytometry?
side scatter
Forward versus side scatter (FSC vs SSC) gating is commonly used to identify cells of interest based on size and granularity (complexity). It is often suggested that forward scatter indicates cell size whereas side scatter relates to the complexity or granularity of the cell.
What is CD45 a marker for?
CD45 is used as a marker of all hematopoietic cells (blood cells), except for mature erythrocytes (red blood cells) and platelets.
What is FSC and SSC in FACS?
One detector measures scatter along the path of the laser (1). This parameter is referred to as forward scatter (FSC). The other detector measures scatter at a ninety degree angle relative to the laser (1). This parameter is called side scatter (SSC).
What does CD45 identify?
CD45 is a receptor linked protein tyrosine phosphatase present in all cells of the hematopoietic lineage except erythrocytes and plasma cells. Originally known as common leukocyte antigen or protein tyrosine phosphatase receptor type C it was given the designation CD45 by the HCDM.
How to select lymphocytes in a cytometry FSC?
They should appear around 20-1000 range . try CSFE “Low”, fsc high, ssc high, live cells (Scatter/dot plot or PI 1:1000)? These values, will be unique for every instrument. I guess that you cannot see a discrete population at all.
How are SSC and FSC used to determine cell size?
Using the FSC and SSC parameters one can look at a mix of cells and distinguish them from one another based off of size and internal complexity. Along the X-axis is the FSC (Forward SCatter) parameter. This parameter is a measurement of the amount of the laser beam that passes around the cell. This gives a relative size for the cell.
How are SSC and FSC density plots used?
A. SSC vs. FSC density plot: B. SSC vs. CD45 PB ( MCA87PB) fluorescence plot. PB, Pacific Blue; FSC, forward scatter; SSC, side scatter. Events can also be displayed as a dot plot where no density information is shown or as a contour map to show the relative intensity of scatter patterns. Examples of contour maps are shown in Figure 14. Fig. 14.
How are SSC and FSC used in Ric?
Using the FSC and SSC parameters one can look at a mix of cells and distinguish them from one another based off of size and internal complexity. Along the X-axis is the FSC (Forware SCatter) parameter. This parameter is a measurement of the amount of the laser beam that passes around the cell.
