How much SYBR is in e Gel agarose?

How much SYBR is in e Gel agarose?

The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide For Research Use Only.

How long does it take agarose gel to cool down?

Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close attention. Let agarose solution cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins.

Why do you need a higher percentage of agarose gel?

A higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger bands. For each sample you want to load on a gel, make 10% more volume than needed because several microliters can be lost in pipetting.

How often do you need to Prime Fortesta gel?

To do so, with the canister in the upright position, slowly and fully depress the actuator eight times. The first 3 actuations may result in no discharge of gel. Safely discard the gel from the first 8 actuations. It is only necessary to prime the pump before the first dose.

How are e-Gel agarose gels used in PCR?

Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, and DNA fragment libraries.

When to use e-Gel pre cast agarose gels?

General information and protocols for using E-Gel pre-cast agarose gels Publication Part Number MAN0000375 Revision A.0 For Research Use Only. Not for use in diagnostic procedures. Information in this document is subject to change without notice.

What is the concentration of ethidium bromide in agarose gel?

® agarose gels contain ethidium bromide, a known mutagen. The concentration of ethidium bromide in each gel ranges from 0.1 to 0.3 µg/mL/. All E- Gel ® agarose gels contain 0.055% Proclin added as a preservative, apart from E-Gel ® 48 4% gels, which contain 0.01% Thimerosal.

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