What wavelength does YFP absorb?
In the Nikon YFP HYQ block, excitation is limited to a 20-nanometer band of wavelengths in the blue-green spectral region (490 to 510 nanometers), and is coupled to an emission filter transmitting yellowish-green fluorescence in the range of 520 to 550 nanometers.
What color is YFP?
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 513 nm and its emission peak is 527 nm.
What is the difference between GFP and YFP?
The main difference between GFP and YFP is that GFP exhibits green color upon exposure to light ranges from blue to ultraviolet whereas YFP exhibits yellow color upon exposure to the same light. Furthermore, GFP is originally derived from the jellyfish, Aequorea Victoria while YFP is a genetic mutant of GFP protein.
Can GFP antibody detect YFP?
YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Aequorea victoria. Recognizes native and denatured forms of jellyfish GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
Can you use GFP and YFP together?
Concurrent use of GFP and YFP could be highly advantageous in many cellular assays, particularly when using cells of transgenic mice whose cells express fluorescent proteins, because generating mice with alternative fluorescent proteins can take a lot of time and effort.
What are the absorption spectra of CFP and YFP?
Figure 1: CFP (donor) and YFP (acceptor) absorption and emission spectra. Overlap between CFP emission and YFP absorption (shaded region) leads to efficient FRET interaction.
What kind of fluorescent protein is eYFP Green?
EYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity. compare Comparison List Add EYFP show comparison clear selection FPbase info
What is the excitation range of YFP HYQ?
In the Nikon YFP HYQ block, excitation is limited to a 20-nanometer band of wavelengths in the blue-green spectral region (490 to 510 nanometers), and is coupled to an emission filter transmitting yellowish-green fluorescence in the range of 520 to 550 nanometers.
What are the excitation and emission spectra of GFP?
Representative excitation (dashed lines) and emission (solid lines) spectra of the three basic GFP variants. Data was derived from reported spectra in the literature. The red-shifted mutants have been developed for use with the standard “fluorescein” filter set and as such utilize the 485/20 excitation and 530/25 emission filter set.
https://www.youtube.com/watch?v=rE41uGTF4fA
