How do you prepare samples for atomic absorption spectroscopy?
A typical sample preparation procedure for solid and viscous liquid samples involves digestion with a concentrated acid; for example, HNO3, HCl, or H2SO4. After dilution of the digested solutions, samples can be directly injected into flame AAS as well as graphite furnace AAS.
What type of samples can the atomic absorption spectroscopy analysis?
6.2. 5 Atomic absorption spectroscopy. AAS is a powerful analytical technique for the analysis of elements present in complex samples such as food and biological and environmental samples by measuring the radiation absorbed by the target element.
How do you prepare a spectroscopy sample?
Sample preparation is the critical step of any analytical protocol, and involves steps from simple dilution to partial or total dissolution. The methods include dry or wet decomposition of the samples in open or closed systems, using thermal, ultrasonic or radiant (infrared, ultraviolet and microwaves) energy.
What acts as a sample container in atomic absorption spectroscopy?
Containers made from transparent parts are used for sampling for trace metals AAS analysis. Plastic containers have their own advantages and disadvantages. However, availability of different chemically modified plastic materials do limit some of the disadvantages.
Which is the most common application of UV / Vis spectroscopy?
By far the most common application in UV/Vis spectrophotometry is the measurement of liquids in cuvettes. However, high performance UV/Vis and UV/Vis/NIR instruments are more commonly used for material’s characterization, which is an area dominated by solid samples.
What are the applications of atomic absorption spectroscopy?
There are many applications of atomic absorption spectroscopy (AAS) due to its specificity. These can be divided into the broad categories of biological analysis, environmental and marine analysis, and geological analysis. Biological samples can include both human tissue samples and food samples.
How is transmittance measured in UV Vis spectroscopy?
This method does not detect the diffuse transmitted light. This measurement method is generally used on thin transparent film or glass not exceeding approximately 3 mm thickness. It is also used to confirm almost 100 % transmittance in a sample with double-sided anti-reflection coatings applied to suppress reflection.
How are complementary colors related in UV spectroscopy?
The remaining light will then assume the complementary color to the wavelength (s) absorbed. This relationship is demonstrated by the color wheel shown on the right. Here, complementary colors are diametrically opposite each other. Thus, absorption of 420-430 nm light renders a substance yellow, and absorption of 500-520 nm light makes it red.