What is DMEM low glucose?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
What is DMEM GlutaMAX?
DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
What is the difference between GlutaMAX and glutamine?
L-glutamine is an essential nutrient in cell cultures for energy production as well as protein and nucleic acid synthesis. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.
What will happen if 50 percent glucose solution is injected intravenously?
Intravenous administration of hypertonic glucose solutions may cause local phlebitis or venous thrombosis and extravasation. If the solution is infused too rapidly, local pain and vein irritation may occur.
Is it safe to take DMEM with high glucose?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
What is in Dulbecco’s modified Eagle’s medium ( DMEM )?
Dulbecco’s Modified Eagle’s Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. This reduced level of sodium bicarbonate (NaHCO 3, 1.5 g/L) is intended for use in 5% CO 2 in air.
Which is the best GIBCO DMEM for cell culture?
Gibco DMEM delivers superior quality, consistency and control for your mammalian cell culture. Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
What kind of cells can be cultured in DMEM?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
How much glucose is in high glucose DMEM?
Specifications
| Glucose Concentration: | 4500 mg/L |
|---|---|
| Volume (Metric): | 500 ml |
| Product Line: | Gibco™ |
| Form: | Liquid |
| Glutamine: | L-Glutamine |
What is DMEM in cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What does DMEM medium contain?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What kind of DMEM is in cellgro complete?
Developed by Mediatech, the cellgro COMPLETE™ formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy’s 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small… Related Products: Cellgro Dmem
Can you use DMEM with high glucose levels?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
What do you need to know about cellgro improved Mem?
Corning® cellgro® Improved MEM (Richter’s Mod.) Thomas No. B003K43 A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites. Complete Certificate of Analysis available for…
What are the uses of cellgro Medium 199?
Corning® cellgro® Medium 199 (Mod.) Medium 199 is often used in vaccine production, virology, and for the culture of various non-transformed cells. It was originally developed as the result of efforts to create a fully defined, animal origin-free medium. Long-term use of this medium requires serum supplementation.
