Why use EDTA free protease inhibitor?
In protein expression and purification protocols, one of the main reasons for the popularity of EDTA free protease inhibitor is because EDTA interferes with Immobilized Metal Chelate Affinity Chromatography. Basically EDTA strips the Nickel ions on purification resins used for binding his-tagged recombinant proteins.
Is EDTA a protease inhibitor?
Description. Protease Inhibitor Cocktail (100X in DMSO, EDTA plus), which is used during cell lysis and protein extraction, is a ready-to-use concentrated stock solutions of multiple protease inhibitors for endogenous protease. EDTA provides inhibition for metalloproteases.
How do protease inhibitors work to stop viruses?
Protease inhibitor drugs block the action of protease enzymes. This prevents protease enzymes from doing their part in allowing HIV to multiply, interrupting the HIV life cycle as a result. This can stop the virus from multiplying.
How are complete mini, EDTA-free protease inhibitor cocktails different?
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating ( e.g. EGTA) agents are included. For life science research only. Not for use in diagnostic procedures. 30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.
Can a metalloproteinase inhibitor be used without EDTA?
• EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations) Pierce Protease Inhibitor Mini Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples.
When to use complete, mini, EDTA-free tablets?
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability ( e.g., metal-containing proteins) or subsequent assays.
What are the features of Pierce protease inhibitor mini tablets?
Features of EDTA-free Protease Inhibitor Mini Tablets: Pierce Protease Inhibitor Mini Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples.